Spectrophotometric activity micro-assay for pure and recombinant cytochrome P450-type nitric oxide reductase Authors

نویسندگان

  • Seike Garny
  • Jan Verschoor
  • Neil Gardiner
  • Justin Jordaan
چکیده

Authors Seike Garny, Jan Verschoor, Neil Gardiner and Justin Jordaan Emerging Health Technologies, Council for Science and Industrial Research in Pretoria, South Africa; Department of Biochemistry, University of Pretoria, South Africa. Short title Nitric oxide reductase activity assay Corresponding author Seike Garny at Molecular Biomaterials, CSIR Biosciences, P.O. Box 395, Pretoria, 0091, South Africa, [email protected], phone number: +27 12 841 3284, fax number: +2712 841 2615 Subject category Enzymatic assays and analyses

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Distinct conformational behaviors of four mammalian dual-flavin reductases (cytochrome P450 reductase, methionine synthase reductase, neuronal nitric oxide synthase, endothelial nitric oxide synthase) determine their unique catalytic profiles.

Multidomain enzymes often rely on large conformational motions to function. However, the conformational setpoints, rates of domain motions and relationships between these parameters and catalytic activity are not well understood. To address this, we determined and compared the conformational setpoints and the rates of conformational switching between closed unreactive and open reactive states i...

متن کامل

Formation of flavin semiquinone during the reduction of P450 BM3 reductase domain with NADPH.

Cytochrome P450 BM3 (P450 102) from Bacillus megaterium is a unique bacterial P450, formed from the fusion of a fatty acid hydroxylase to a eukaryotic-like NADPH-cytochrome P450 reductase flavoprotein in a single (1 19 kDa) polypeptide chain (1, 2). It is an attractive model system for enzymological and structural studies due to its homology with mammalian drug metabolising P450 systems, and wi...

متن کامل

Dissection of NADPH-cytochrome P450 oxidoreductase into distinct functional domains.

NADPH-cytochrome P450 oxidoreductase transfers electrons from NADPH to cytochrome P450 and catalyzes the one-electron reduction of many drugs and foreign compounds. This enzyme is a flavoprotein containing the cofactors FMN and FAD, which are essential for its function. We have expressed the putative FMN and FAD/NADPH binding domains of P450 reductase and show that these distinct peptides fold ...

متن کامل

Correlation between Cytochrome P450, 5-alpha Reductase, and Androgen Receptor Levels in Patients with Type 2 Diabetes Mellitus

Objective: Type 2 diabetes mellitus (T2DM) is one of the most common chronic diseases. The CYP450 plays an important role in the biosynthesis of steroid hormones and the hormonal activity is mediated by the androgen receptor (AR) and the enzyme 5-alpha reductase (5αR). Therefore, this study aimed to investigate the relationship between these factors in T2DM. Materials and Methods: This case-co...

متن کامل

Down-regulation of cytochrome P450 mRNAs and proteins in mice lacking a functional NOS2 gene.

Endotoxemia results in both the down-regulation of multiple cytochrome P450 genes and the induction of inducible nitric oxide synthase (NOS2). The nitric oxide (NO) released during inflammation has been implicated as the mediator of the decreased catalytic activity and expression of several cytochrome P450 isozymes. We examined the role of NO in the decreases in both gene expression and activit...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2014